WebFig. 31. Antibody titration. Plotting the stain index for each concentration of antibody will allow you to titrate the optimal amount of antibody for your experiment. For more in-depth information on Antibody Titration see our Antibody Titration in Flow Cytometry page . Useful Tools There are useful tools that can help with panel design. WebFlow Cytometry Resource Guide. BioLegend is a globally recognized leader in flow cytometry. To advance the capabilities of this application, we provide researchers with …
BioLegend hiring Product Manager - Cell Analysis in San Diego ...
WebThe Product Manager, Cell Analysis will focus on supporting customer, product and commercial objectives for BioLegend’s Cell Analysis portfolio, which includes flow cytometry antibodies and ... WebTotalSeq™-C antibodies can be used for Cell Hashing in the 5' workflow, and can be purchased from BioLegend directly. We strongly recommend that you reach out directly to BioLegend technical support ([email protected]) for recommendation for antibodies, etc prior to initiating your experiments. chaz you pull it
Cell Surface - BD Biosciences
WebJan 1, 2024 · Here, we show how flow cytometry can be used to track changes in mitochondrial mass, membrane potential and superoxide (ROS) production in live immune cells. This protocol suggests a quick way of evaluating mitochondrial fitness using flow cytometry. We propose using the probes MitoTraker Green and MitoTracker Red/ … WebVikingson, A., K. Pederson and D. Muller. 1994. Enumeration of IFN-gproducing lymphocytes by flow cytometry and correlation with quantitative measurement of IFN-g. J. Immunol. Meth. 173:219-228. Prussin, C. and D. Metcalfe. 1995. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine … WebAdd antibodies to cells and incubate for 20 minutes on ice, protected from light. Wash the cells two times with either 200-µL (for plates) or 1-mL (for tubes) volumes of Stain Buffer. Centrifuge cells at 300 g for 5 minutes. Carefully aspirate (for microwell plates or tubes) or invert and blot away (for tubes) supernatants from cell pellets. chazy orchards.com