Dethiobiotin synthase

Author: vbbv

August undefined, 2024

WebDethiobiotin. The conversion of dethiobiotin (4) into biotin (5), which is catalyzed by biotin synthase, is the most novel step in biotin biosynthesis since it involves the formal … WebMar 3, 1998 · HPLC analysis of reaction mixtures revealed the presence of a compound with the characteristics of an intermediate: (1) it was labelled with 14C, and therefore derived from the [14C]dethiobiotin substrate; (2) it was present only in reaction mixtures containing biotin synthase; (3) it was not derived from [14C]biotin; (4) 35S from [35S]cystine ...

Dethiobiotin: Uses, Interactions, Mechanism of Action - DrugBank

Webwere included. See Data S1 for genomic and taxonomic data. Author: 15716_sing Created Date: 03/23/2024 23:15:00 Last modified by: 15716_sing WebAug 17, 2024 · Mycobacterium tuberculosis dethiobiotin synthase ( Mt DTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. … campervan hire hawick

Information on EC 6.3.3.3 - dethiobiotin synthase

WebJan 2, 2004 · The crystal structure of biotin synthase from Escherichia coli in complex with S-adenosyl-L-methionine and dethiobiotin has been determined to 3.4 angstrom resolution.This structure addresses how “AdoMet radical” or “radical SAM” enzymes use Fe 4 S 4 clusters and S-adenosyl-L-methionine to generate organic radicals.Biotin synthase … WebGSE12663: virulence determinants of Francisella tularensis revealed by transcriptional profiling inside macrophages: GSE42863: Flagellar export apparatus protein, FliH, is involved in post-transcriptional regulation of FlaB, motility and virulence of the relapsing fever spirochete Borrelia hermsii Webdethiobiotin synthetase, dethiobiotin synthase, more top print hide 6 entries Go to Reaction Search REACTION REACTION DIAGRAM COMMENTARY ORGANISM … camper van hire gloucestershire

d-Desthiobiotin = 98 TLC 533-48-2 - Sigma-Aldrich

Biotin synthase from Escherichia coli: isolation of an enzyme …

WebMay 1, 2005 · Biotin synthase is an S-adenosyl-L-methionine (SAM) radical enzyme that inserts sulfur into dethiobiotin to produce biotin. The reaction proceeds through 5′-deoxyadenosyl radical intermediates that become reduced during the sulfur insertion step to give another product of the reaction, 5′-deoxyadenosine. We report that Escherichia coli … WebMar 9, 2024 · biotin synthase. GeneRIFs: Gene References Into Functions. In biotin synthase, abstraction of a hydrogen atom from the C9 methyl group of dethiobiotin results in formation of a dethiobiotinyl methylene carbon radical, which is quenched by a sulfur atom to form a new carbon-sulfur bond in 9-mercaptodethiobiotin. camper van hire hawkes bayWebJan 13, 1994 · Strands 1, 4, 5, and 6 form the classic mononucleotide-binding motif as found in other ATP- and GTP-binding proteins. Dethiobiotin synthase from E. coli is a dimer built up of two identical subunits. The dimer is formed through packing along a molecular two-fold axis, which in the crystals coincides with the crystallographic two-fold axis. campervan hire henfield

"WebMar 28, 1995 · One of these enzymes, ATP-dependent dethiobiotin synthetase, catalyzes the carboxylation of 7,8-diaminopelargonic acid leading to the formation of the ureido ring of biotin. The enzyme belongs to the class of ATP-dependent carboxylases and we present here the first crystal structure determined for this class of enzyme. " - Dethiobiotin synthase

Dethiobiotin synthase

WebSep 5, 1995 · Dethiobiotin Synthetase: The Carbonylation of 7,8-Diaminononanoic Acid Proceeds Regiospecifically via the N7-Carbamate. Biochemistry 1995, 34 (35) , 10976-10984.

Did you know?

WebBiotin synthase (BioB) catalyzes the oxidative insertion of a sulfur atom between the C6 methylene and the C9 methyl positions in dethiobiotin. The enzyme couples oxidation … WebJan 11, 2024 · Biotin synthase belongs to the radical SAM superfamily and contains the signature Cys-X-X-X-Cys-X-X-Cys motif sequence required for [4Fe-4S] cluster binding. …

WebJun 13, 2005 · Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism. Gene Name bioB Uniprot … WebMycobacterium tuberculosis dethiobiotin synthase (MtDTBS) is a crucial enzyme involved in the biosynthesis of biotin in the causative agent of tuberculosis, M. tuberculosis. …

WebJul 1, 2007 · KAPA synthase from A. thaliana was localized in the cytosol by confocal microscopy of the GFP-fused enzyme and by Western blotting (Pinon et al., 2005). Sequence homologs of the bacterial 7,8-diaminopelargonic acid (DAPA) aminotransferase and dethiobiotin synthase exist in the A. thaliana genome (Pinon et al., 2005). WebAug 21, 2012 · DAPA synthase and dethiobiotin synthase are encoded by adjacent genes that can be alternatively spliced to specify a bifunctional fusion protein or separate enzymes (Muralla et al., 2008). The fusion and the separate dethiobiotin synthase are predicted to be mitochondrial and the separate DAPA synthase is predicted to be cytosolic; …

In enzymology, a dethiobiotin synthase (EC 6.3.3.3) is an enzyme that catalyzes the chemical reaction ATP + 7,8-diaminononanoate + CO2 ADP + phosphate + dethiobiotin The 3 substrates of this enzyme are ATP, 7,8-diaminononanoate, and CO2, whereas its 3 products are ADP, phosphate, and dethiobiotin.

WebJan 23, 2007 · Dethiobiotin synthase 1 UniRule annotation. Gene names. Name. bioD1 UniRule annotation. Ordered locus names. b0778, JW0761. Organism names. Organism. Escherichia coli (strain K12) Taxonomic identifier. 83333 NCBI. Taxonomic lineage. Bacteria > Proteobacteria > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > … camper van hire hertsWebEC Tree. 6 Ligases. 6.3 Forming carbon-nitrogen bonds. 6.3.3 Cyclo-ligases. 6.3.3.3 dethiobiotin synthase. IUBMB Comments. CTP has half the activity of ATP. Specify your search results. Mark a special word or phrase in this record: first theater in the philippinesWebThis article is published in Journal of the American Chemical Society.The article was published on 1952-06-01. It has received 43 citation(s) till now. first theater in englandWebBiotin synthase, a member of the ‘radical SAM’ (S-adenosylmethionine) family, converts DTB (dethiobiotin) into biotin. The active form of the Escherichia coli enzyme contains two (Fe-S ... first theater in londonWebOct 15, 1998 · The crystal structure of the 224-residue protein dethiobiotin synthetase from Escherichia coli has been refined using X-ray diffraction data at 0.97 A resolution at 100 K. The model, consisting of 4143 protein atoms including 1859 H atoms and 436 solvent sites, was refined to a final R factor of 11.6% for all reflections, and has an estimated ... campervan hire hervey bayWebBiotin synthase catalyzes formation of the thiophane ring through stepwise substitution of a sulfur atom for hydrogen atoms at the C9 and C6 positions of dethiobiotin. Biotin synthase is a radical S-adenosylmethionine (SAM) enzyme that reductively cleaves S-adenosylmethionine, generating 5′-deoxyadenosyl radicals that initially abstract a … campervan hire hervey bay qldWebNov 18, 2005 · Working model for the compartmentation of biotin metabolism in the plant cell. Biotin-synthesizing enzymes are as follows: 1, KAPA synthase; 2, DAPA aminotransferase; 3, dethiobiotin synthase; and 4, biotin synthase. The occurrence of reaction 2 in the cytosol and of reaction 3 in mitochondria is predicted from DNA … camper van hire hinckley