WebDec 22, 1998 · The heme domain of FixL regulates a histidine kinase domain that transduces its signal by a conserved phosphoryl transfer mechanism (5, 6).Deoxy-FixL (Fe 2+), but not oxy-FixL (Fe +2 O 2), autophosphorylates at a histidine with a γ-phosphate from ATP.In Rhizobia, the transfer of this phosphoryl group to the transcription factor FixJ …
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WebNov 1, 1995 · The R. leguminosarum bv. phaseoli FixL protein does not contain the heme-binding motif defined for the previously described FixL proteins. R. leguminosarum bv. phaseoli fixLJ and fixJ mutants were constructed. These mutants can still fix nitrogen, albeit at a reduced level. Expression analysis of nifA-gusA and nifH-gusA fusions in the ... WebThe sensor kinase FixL and the response regulator FixJ induce the expression of the nitrogen fixation genes of Rhizobium meliloti in response to microaerobiosis, which is a characteristic feature of the plant root nodule interior where the bacteria fix nitrogen. The kinase activity of a purified, soluble derivative of the membrane-bound ... can olive oil be toxic
(PDF) Autophosphorylation and phosphatase activities of the …
Webof FixL* from E. coli strainTG1 bearingpGG820 and plasmid pCHK57 has been described (16). The purification of FixJ from TG1 bear ing pCHK57 and pMW5 has been described (29). FixL*H138Q, FixL*H194N, and FixL*H285Q were purified exactly as was FixL*, except that the strain used for overexpression of these three plasmids was BL21 bearing … WebMay 4, 2001 · The FixL/FixJ two-component regulatory system of Sinorhizobium meliloti controls the expression of nitrogen fixation genes in response to O 2.When phosphorylated, the transcription factor FixJ binds to the nifA and fixK promoters in S. meliloti and induces expression of the corresponding genes, both of which encode key transcription … WebApr 10, 2024 · The FixL and FixJ two-component system (TCS) of this bacterium ensures that nitrogen fixation is only stimulated under conditions of low oxygen. When it is not … can olive oil be used on granite