WitrynaDocuments. Gibco™ Glucose Solution contains D-glucose at a concentration of 200 g/L and is used as a serum-free medium supplement suitable for cell culture. Glucose … WitrynaPromotional Article Monitoring. Register your specific details and specific drugs of interest and we will match the information you provide to articles from our extensive database and email PDF copies to you promptly.
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WitrynaIMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a … IMDM - IMDM - Thermo Fisher Scientific Witryna4 kwi 2024 · All cell lines were maintained in the IMDM medium (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS and 1% penicillin-streptomycin (Thermo Fisher Scientific) and tested for mycoplasma contamination (MycoAlert, Lonza). Establishment of the IRAK3-KO mouse model using CRISPR/Cas9.
WitrynaIMDM media. Gibco Iscove’s Modified Dulbecco’s Medium (IMDM) is a highly enriched medium well suited for rapidly proliferating, high-density cell cultures, including Jurkat, … WitrynaIn total, 300 MSC were seeded on 6-well culture plates (TPP, Switzerland) in IMDM with 15% FBS. The medium was changed on the fifth day of culture. ... (ThermoFisher, USA) and anti-βIII-tubulin (1:800) (Santa Cruz) antibodies at room temperature for one hour. The samples were washed and incubated with a secondary anti-rabbit TRITC (1:350 ...
WitrynaLive cells were measured with Countess II Automated Cell Counters (ThermoFisher Scientific, Waltham, MA, USA) for each condition at the end of the cell culture. Unstimulated samples had a viability ≥80%, while stimulated samples had a viability between 70 and 80%. ... Previous studies compared IMDM (Iscove’s modified … WitrynaIMDM (Iscove's Modified Dulbecco's Medium) is well suited for rapidly proliferating, high-density cell cultures, including Jurkat, COS-7, and macrophage cells. We offer a …
Witryna28 lis 2024 · In this experiment, Trizol (#254712, Invitrogen, Thermo Fisher Scientific, USA) was added to lyse the cells for the RNA extraction, and the RNase-Free DNase І system (M6101, Promega, USA) was used to remove genomic DNA. The purity of total RNA was determined by an Eppendorf BioSpectrometer (Eppendorf, Hamburg, …
Witryna10 paź 2024 · Cell culture and Phorbol 12-myristate 13-acetate (PMA) stimulation of cells. MV4-11 and AML193 cells were obtained from ATCC and were cultured in Iscove's Modified Dulbecco's Medium (IMDM) (ThermoFisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS), 5 mM L-glutamine, 1% … openshift create podWitrynaKATO-III cells were cultured in 80% IMDM (ATCC, Manassas, VA, USA) supplemented with 20% FBS (Gibco, Waltham, MA, USA). The other cell lines were maintained in RPMI-1640 medium replenished with 10% FBS. ... (Thermo Fisher Scientific, Waltham, MA, USA) and was converted into cDNA using the PrimeScript RT Reagent Kit … openshift container platform youtubeWitryna10 kwi 2024 · The cells in the culture supernatant were collected at the end of step 2 and incubated with the specific medium for final erythroid maturation, based on the protocol by Huang et al. , composed of Iscove’s Modified Dulbecco’s Medium (IMDM) (Thermo Fisher Scientific) supplemented with EPO (3 U/mL), holo-transferrin (500 µg/mL), … openshift delete completed podsWitryna10 kwi 2024 · The feeder cells were isolated from spleen as described above, expanded with CD3/CD28 beads in IMDM with 100 ng ml −1 mouse interleukin 2 for 3 days and received 30–35 Gy irradiation through a ... openshift dc bcWitryna7 kwi 2024 · After two days, the suspension culture of core spheroids was then replaced by human placental microvascular pericytes (Angio-Proteomie) suspension at a density of 7.5 × 10 4 cells/mL in the IMDM ... openshift copy file from podWitryna27 gru 2024 · 类似的情况还发生在另一个巨头赛默飞（www.thermofisher.com）身上，其实对赛默飞这样的医疗巨头来说，科研只是很小的一块市场，小到不足其公司市值的十分之一。但特别是在近两年，不论是其兼并收购、强化直销还是大范围的品牌推广，都可以看得出来像Sigma和 ... openshift delete evicted podsWitrynaCells were grown in IMDM medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% Fetal Bovine Serum (FBS; Thermo Fisher Scientific), 2mM L-glutamine, and 100U/mL penicillin, 100µg/mL streptomycin at 37 °C in a humidified incubator containing 5% CO 2. All cell lines used in the study were approved by the … i paid it in spanish