Irf3 phosphorylation antibody
WebAll mutants lacked phosphorylation of the STING-S365 residue, IRF3, and STAT1 upon stimulation, indicating defects in IRF3 activation and type I IFN signaling (Fig. 1A and SI Appendix, Fig. S1A). Consistently, all mutants were deficient in IRF3 nuclear translocation ( Fig. 1 B and SI Appendix , Fig. S1 B ) and did not produce IFNβ after DMXAA ... WebMar 21, 2024 · IRF3 (Interferon Regulatory Factor 3) is a Protein Coding gene. Diseases associated with IRF3 include Encephalopathy, Acute, Infection-Induced 7 and Encephalopathy, Acute, Infection-Induced 10 . Among its related pathways are Cytosolic sensors of pathogen-associated DNA and MyD88 dependent cascade initiated on …
Irf3 phosphorylation antibody
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WebPhospho-IRF-3 (Ser396) Antibody detects endogenous levels of IRF-3 only when phosphorylated at Ser396. This antibody does not cross-react with other family members at physiological conditions. Species Reactivity: Human. Species predicted to react based on … Highly specific and rigorously validated in-house, Phospho-IRF-3 (Ser396) (4D4G) … WebNational Center for Biotechnology Information
Web(C,D) 4b(Δ2-38) inhibits the phosphorylation and nuclear translocation of IRF3. Experiments were carried out as in AB, except that 4b(Δ2-38) was accessed. Source publication WebFigure Legend Snippet: TAOK1 promotes TBK1-IRF3 complex formation. a HEK293T cells were transfected with plasmids encoding HA-TBK1, Flag-IRF3, and varying doses of a plasmid encoding Myc-TAOK1 (0.5, 1.0, and 1.5 μg). Cells were harvested 24 h after transfection for immunoblot analysis as indicated with the antibody to Flag tag. b …
WebUse Anti-phospho-IRF-3 (Ser396) Antibody (Rabbit Polyclonal Antibody) validated in WB to detect phospho-IRF-3 (Ser396) also known as Interferon regulatory factor 3. Anti-phospho … WebThe induction of phosphorylation of STING on S366 of IRF3 on S396 was inhibited in T404E compared to WT or ... T404A, or T404E STAT2 were treated with cisplatin for 24 h. The cells were fixed then stained with IRF3 antibody (red) and DAPI for confocal analysis. (C) STAT2-deficient Calu-1 cells restored with WT, T404A, T404E, or Y690F STAT2 ...
WebMar 12, 2015 · IRF3 activation requires phosphorylation, dimerization and nuclear translocation. However, the mechanisms for the termination of IRF3 activation in nucleus are elusive. Here we report the identification of TRIM26 to negatively regulate IFN-β production and antiviral response by targeting nuclear IRF3.
WebDec 15, 2014 · To directly observe the distribution of HSPD1 and activated IRF3, an antibody specific to phosphorylated IRF3 (phospho S386) was used. Not surprisingly, overexpression of MAVS for 16 h resulted in phosphorylation of IRF3. Furthermore, almost all of the activated IRF3 co-localized with HSPD1 at that time point . open pitched roofopen pit bathroom deathWebWestern blot analysis of Phospho-IRF3 pSer385 using Phospho-IRF3 pSer385 polyclonal antibody (Product # PA5-36775) at a dilution of 1:500. Lane 1: Hela cell lysate treated with PMA (100nM, 15min), Lane 2: … ipad pro 2021 11 inch refurbishedWebKey features and details Rabbit polyclonal to IRF3 Suitable for: WB Reacts with: Human Isotype: IgG You may also be interested in Western blot … ipad pro 2021 11 inch case with pencil holderWebHere we show that STING stimulates IRF3 phosphorylation by the kinase TBK1 in an in vitro reconstitution system. Using this system, we identified a carboxyl terminal region of STING that is both necessary and sufficient to activate TBK1 and stimulate IRF3 phosphorylation. open pit and geotechnical engineer and africaWebMar 5, 2024 · Rnf39 deficiency and knockdown both enhanced RNA virus–induced IRF3 phosphorylation (Fig. 2, A to C, and fig. S3D). Furthermore, ... The cell lysates were immunoprecipitated with Flag antibody and then immunoblotted with the indicated antibodies. The specific bands were marked by asterisk. All experiments were repeated at … ipad pro 2021 charger wattageWebJan 19, 2024 · The phosphorylation of IRF3 plays critical roles in determining the immune state and the cell fate against viral infection. Until now, the sequential activation mode of IRF3 through multi-site phosphorylation remains controversial. ... Antibodies; Rabbit polyclonal anti-IRF3: Santa Cruz Biotechnology: Cat# sc-9082; RRID: AB_2264929: Anti … open pit bbq chips